Testing (troubleshooting) protocolWe kindly ask you to fill out this form. It is required for our ISO certification process and it helps us tremendously to see, how we are doing. Thank you very much in advance.

Antibody - sample
Antibody name:
Cat. #:
Lot #:
Clone #:
Testing date:

Testing laboratory
Laboratory name:
Laboratory address:
Person responsible for testing:
E-mail contact:
Person responsible for evaluation:  (e.g. Pathologist)
E-mail contact:

Antibody - sample
Tested material (very important): (type of the tissue; type of the fixative; method of fixation; time of fixation; deparaffinization; rehydration; please, specify the solutions and times used for both)
Staining system:	Ventana Benchmark DAKO Autostainer Lab Vision Autostainer Bond Autostainer Manual Other
	Please, specify if "Other" was selected:

Pretreatment / Revitalization
HIER	Time	In
Citrat, pH6 TRIS/EDTA pH9 EDTA pH8 Other	5 min. 10 min. 20 min. Other	microwave water bath pressure cooker Other
Please, specify if "Other" was selected:	Please, specify if "Other" was selected:	Please, specify if "Other" was selected:

Pretreatment / Revitalization temperature:
Cooled in revitalization buffer:	Yes No	(if Yes) please fill the time in minutes:

PIER		Temperature		Time
Trypsin (0,1%, pH 7,2) Pronase (0,05%, pH 7,2) Pepsin (0,1%, pH 2,5) Other		37° C RT (cca 22° C)		5 min. 10 min. 20 min. Other
Please, specify if "Other" was selected:				Please, specify if "Other" was selected:

Blocking of Endogenous Peroxidase:	Yes No
	(if Yes) % of H2O2 used:
	(if Yes) Incubation time:

Protein block:	Yes No
	(if Yes) Type:
	(if Yes) Time:

Washing buffer
Type of Washing buffer:
pH:
Presence of the detergent (Tween; Triton) in washing buffer:	Yes No
Time of washing (in minutes):

Primary antibody
Incubation time (in minutes):	30 min. 60 min. 120 min. overnight Other
	Please, specify if "Other" was selected:
Incubation temperature:	RT (cca 22° C) 37° C 4-8° C
Dilution:	RTU 1:10 1:20 1:50 1:100 1:200 1:300 1:400 1:500 1:800 1:1000 1:1500 Other
	Please, specify if "Other" was selected:
What kind of primary antibody diluent was used:

Additional information
Secondary antibody:
Detection system:
Detection system with amplifier:	Yes No
Time of incubation with detection system (in minutes):
Enzyme:	HRP AP
Chromogen:	AEC DAB Perm.Red DAKO Red Other
	Please, specify if "Other" was selected:
Time of incubation with the chromogen (in minutes):
Comments (please, briefly describe the problem; e.g. non-specific staining; stained background; no staining at all, etc.):

Please, attach the pictures of the stained tissue. Very important!
Please, send us some stained pictures. Max file size 3MB.	Upload:Upload file
	Upload:Upload file
	Upload:Upload file