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| Cat. #: |
DB 002 |
| Volume: |
100 μl |
| Dilution: |
WB - 1:2 000, ELISA - 1:10 000 - 1:20 000 |
| Application: |
WB, ELISA, IP |
| Availability: |
Available immediately |
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Datasheet
| Product description: |
Rabbit anti-p53 clonal IgGs. |
| Immunogen: |
Peptide derived from C-terminal peptide of human p53. |
| Species Reactivity: |
Human, rat, mouse-tested. |
| Basic information: |
Major clone of IgGs obtained from immunoaffinity purified
immunoglobulins corresponding to immunogenic peptide. |
| Buffer: |
20 mM Tris-HCl, pH 8.0 |
| Stabilizer: |
10 mg/ml BSA. |
| Preservative: |
0.05% Sodium Azide. |
| Storage: |
10 μl aliquots at -20° C. |
| Handling: |
Avoid repeated freezing and thawing. |
| Expiration: |
18 months (from the date of delivery). |
| Applications: |
Western blot (transfer to PVDF membrane is recommended), Immunoprecipitation, ELISA, Immunohistochemistry (frozen sections, fixed, paraffin-embedded sections) |
| Dilution range: |
Western blotting – 1:2,000. ELISA – 1:10,000-1:20,000. Immunoprecipitation, immunohistochemistry – to be tested by user. |
| Western immunoblotting solutions: |
- Wash buffer: 1x Tris Buffered Saline (TBS); 0.2% Tween-20
- Blocking buffer: 1xTBS; 0.2% Tween 20; 5% nonfat dry milk |
| For western blots, incubate the membrane with antibody diluted in blocking buffer 2 hours at room temperature. |
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