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| Cat. #: |
DB 013 |
| Volume: |
100 µl |
| Dilution: |
WB - 1:5 000, ELISA - 1:20 000 - 1:100 000 |
| Application: |
WB, ELISA, IP |
| Availability: |
Available immediately |
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Datasheet
| Product description: |
Rabbit anti - phospho - Erk 1,2 clonal IgGs. |
| Immunogen: |
Peptide derived from the protein area including conserved pT-E-pY motif of activated Erk 1,2. |
| Species Reactivity: |
Human, rat, mouse-tested. |
| Basic information: |
Major clone of IgGs representing both, Erk1 and Erk2, obtained from immunoaffinity purified immunoglobulins corresponding to pT-E-pY dual phosphorylation sequence. |
| Buffer: |
20 mM Tris-HCl, pH 8.0. |
| Stabilizer: |
10 mg/ml BSA. |
| Preservative: |
0.05% Sodium Azide. |
| Storage: |
10 μl aliquots at -20° C. |
| Handling: |
Avoid repeated freezing and thawing. |
| Expiration: |
18 months (from the date of delivery). |
| Applications: |
Western blot, Immunoprecipitation, ELISA, Immunohistochemistry (frozen sections, fixed, paraffin-embedded sections). |
| Dilution range: |
Western blotting – 1:5,000. ELISA – 1:20,000-1:100,000. Immunoprecipitation, immunohistochemistry – to be tested by user. |
| Western immunoblotting solutions: |
- Wash buffer: 1x Tris Buffered Saline (TBS); 0.1% Triton X-100.
- Blocking buffer: 1xTBS; 0.1% Triton X-100; 5% BSA (used with the primary antibody). |
| For western blots, incubate the membrane with antibody diluted in blocking buffer 2 hours at room temperature. |
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