|
|
|
|
|
100 μl
WB - 1:5 000, ELISA - 1:50 000 - 1:100 000
Datasheets in PDF format to download
Datasheet
| Product description: |
Rabbit anti - nephrin clonal IgGs. |
| Immunogen: |
Peptide derived from the centre of the fifth Ig-like domain of human nephrin (between residues Cys465-Cys528). |
| Species Reactivity: |
Human, rat, mouse-tested. |
| Basic information: |
Major clone of IgGs obtained from immunoaffinity purified immunoglobulins corresponding to immunogenic peptide. |
| Buffer: |
20 mM Tris-HCl, pH 8.0. |
| Stabilizer: |
10 mg/ml BSA. |
| Preservative: |
0.05% Sodium Azide. |
| Storage: |
10 μl aliquots at -20° C. |
| Handling: |
Avoid repeated freezing and thawing. |
| Expiration: |
18 months (from the date of delivery). |
| Applications: |
Western blot, ELISA, Immunoprecipitation, Immunohistochemistry (IHC-P). |
| Dilution range: |
Western blotting – 1:5,000. ELISA – 1:50,000-1:100,000. IHC-P - 1:200. |
| Western immunoblotting solutions: |
- Wash buffer: 1x Tris Buffered Saline (TBS); 0.1% Triton X-100
- Blocking buffer: 1xTBS; 0.1% Triton X-100; 5% nonfat dry milk |
| For western blots, incubate the membrane with antibody diluted in blocking buffer 2 hours at room temperature. |
|