DB Biotech, spol. s r.o.

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Anti - CA IX (Carbonic Anhydrase IX) – pT443 specific		Add to price request cart
Cat. #:	DB 016
Volume:	100 μl
Dilution:	WB - 1:2 000, ELISA - 1:25 000
Application:	WB, ELISA, IP
Availability:	Available immediately
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Datasheet

Product description:	Rabbit anti-CA IX, pT443-specific clonal IgGs.
Immunogen:	Peptide corresponding to the sequence surrounding the phosphorylated Thr443 residue.
Species Reactivity:	Human.
Basic information:	Major clone of IgGs obtained from immunoaffinity purified immunoglobulins corresponding to immunogenic peptide.
Buffer:	20 mM Tris-HCl, pH 8.0.
Stabilizer:	10 mg/ml BSA.
Preservative:	0.05% Sodium Azide.
Storage:	10 μl aliquots at -20° C.
Handling:	Avoid repeated freezing and thawing.
Expiration:	18 months (from the date of delivery).
Applications:	Western blot, ELISA.
Dilution range:	Western blotting – 1:2,000. ELISA – 1:25,000.
Western immunoblotting solutions:	- Wash buffer: 1x Tris Buffered Saline (TBS); 0.2% Triton X-100 - Blocking buffer: 1xTBS; 0.2% Triton X-100; 8% nonfat dry milk
For western blots, incubate the membrane with antibody diluted in blocking buffer 2 hours at room temperature.